Počet záznamů: 1
Regulation of Isoprenoid Pheromone Biosynthesis in Bumblebee Males
- 1.0458703 - ÚOCHB 2017 RIV DE eng J - Článek v odborném periodiku
Prchalová, Darina - Buček, Aleš - Brabcová, Jana - Žáček, Petr - Kindl, Jiří - Valterová, Irena - Pichová, Iva
Regulation of Isoprenoid Pheromone Biosynthesis in Bumblebee Males.
Chembiochem. Roč. 17, č. 3 (2016), s. 260-267. ISSN 1439-4227. E-ISSN 1439-7633
Grant CEP: GA MŠMT LO1302; GA ČR GA15-06569S
Institucionální podpora: RVO:61388963
Klíčová slova: biosynthesis * Bombus spp. * gene expression * isoprenoids * pheromones * transcriptional regulation
Kód oboru RIV: CE - Biochemie
Impakt faktor: 2.847, rok: 2016
Males of the closely related species Bombus terrestris and Bombus lucorum attract conspecific females by completely different marking pheromones. MP of B.terrestris and B.lucorum pheromones contain mainly isoprenoid (ISP) compounds and fatty acid derivatives, respectively. Here, we studied the regulation of ISP biosynthesis in both bumblebees. RNA-seq and qRT-PCR analyses indicated that acetoacetyl-CoA thiolase (AACT), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR), and farnesyl diphosphate synthase (FPPS) transcripts are abundant in the B.terrestris labial gland. Maximal abundance of these transcripts correlated well with AACT enzymatic activity detected in the LG extracts. In contrast, transcript abundances of AACT, HMGR, and FPPS in B.lucorum were low, and AACT activity was not detected in LGs. These results suggest that transcriptional regulation plays a key role in the control of ISP biosynthetic gene expression and ISP pheromone biosynthesis in bumblebee males.
Trvalý link: http://hdl.handle.net/11104/0258951
Počet záznamů: 1