Počet záznamů: 1
Prolyl Isomerase Pin1 Regulates Axon Guidance by Stabilizing CRMP2A Selectively in Distal Axons
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SYSNO ASEP 0455250 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Prolyl Isomerase Pin1 Regulates Axon Guidance by Stabilizing CRMP2A Selectively in Distal Axons Tvůrce(i) Balaštík, Martin (FGU-C) RID, ORCID
Zhou, X.Z. (US)
Alberich-Jorda, M. (US)
Weissová, Romana (FGU-C) ORCID, RID, SAI
Žiak, Jakub (FGU-C) RID, ORCID
Pazyra-Murphy, M.F. (US)
Cosker, K.E. (US)
Machoňová, O. (CZ)
Kozmiková, I. (CZ)
Chen, CH. (US)
Pastorino, L. (US)
Asara, J.M. (US)
Cole, A. (GB)
Sutherland, C. (GB)
Segal, R. A. (US)
Lu, K.P. (US)Zdroj.dok. Cell Reports. - : Cell Press - ISSN 2211-1247
Roč. 13, č. 4 (2015), s. 812-828Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Pin1 ; axon guidance ; Semaphorin 3A Vědní obor RIV ED - Fyziologie Institucionální podpora FGU-C - RVO:67985823 UT WOS 000363780900016 EID SCOPUS 84945540482 DOI https://doi.org/10.1016/j.celrep.2015.09.026 Anotace Axon guidance relies on precise translation of extracellular signal gradients into local changes in cytoskeletal dynamics, but the molecular mechanisms regulating dose-dependent responses of growth cones are still poorly understood. Here, we show that during embryonic development in growing axons, a low level of Semaphorin3A stimulation is buffered by the prolyl isomerase Pin1. We demonstrate that Pin1 stabilizes CDK5-phosphorylated CRMP2A, the major isoform of CRMP2 in distal axons. Consequently, Pin1 knockdown or knockout reduces CRMP2A levels specifically in distal axons and inhibits axon growth, which can be fully rescued by Pin1 or CRMP2A expression. Moreover, Pin1 knockdown or knockout increases sensitivity to Sema3A-induced growth cone collapse in vitro and in vivo, leading to developmental abnormalities in axon guidance. These results identify an important isoform-specific function and regulation of CRMP2A in controlling axon growth and uncover Pin1-catalyzed prolyl isomerization as a regulatory mechanism in axon guidance Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2016
Počet záznamů: 1