Mechanism of photoprotection in the cyanobacterial ancestor of plant antenna proteins

Staleva, H.; Komenda, Josef; Shukla, Mahendra K.; Šlouf, V.; Kaňa, Radek; Polívka, Tomáš; Sobotka, Roman

doi:https://dx.doi.org/10.1038/nchembio.1755

Počet záznamů: 1

Mechanism of photoprotection in the cyanobacterial ancestor of plant antenna proteins

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SYSNO ASEP	0447354
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Mechanism of photoprotection in the cyanobacterial ancestor of plant antenna proteins
Tvůrce(i)	Staleva, H. (CZ) Komenda, Josef (MBU-M)_{RID, ORCID} Shukla, Mahendra K. (MBU-M)_RID Šlouf, V. (CZ) Kaňa, Radek (MBU-M)_{RID, ORCID} Polívka, Tomáš (BC-A)_{RID, ORCID} Sobotka, Roman (MBU-M)_{RID, ORCID}
Zdroj.dok.	Nature Chemical Biology . - : Nature Publishing Group - ISSN 1552-4450 Roč. 11, č. 4 (2015), s. 287-291
Poč.str.	5 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	photosystem II ; fluorescence ; chlorophyll
Vědní obor RIV	BO - Biofyzika
Vědní obor RIV – spolupráce	Biologické centrum (od r. 2006) - Biofyzika
CEP	GBP501/12/G055 GA ČR - Grantová agentura ČR
	GA14-13967S GA ČR - Grantová agentura ČR
	ED2.1.00/03.0110 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Institucionální podpora	MBU-M - RVO:61388971 ; BC-A - RVO:60077344
UT WOS	000351666500012
DOI	https://doi.org/10.1038/nchembio.1755
Anotace	Plants collect light for photosynthesis using light-harvesting complexes (LHCs)-an array of chlorophyll proteins that are able to reversibly switch from harvesting to energy-dissipation mode to prevent damage of the photosynthetic apparatus. LHC antennae as well as other members of the LHC superfamily evolved from cyanobacterial ancestors called high light-inducible proteins (Hlips). Here, we characterized a purified Hlip family member HliD isolated from the cyanobacterium Synechocystis sp. PCC 6803. We found that the HliD binds chlorophyll-a (Chl-a) and beta-carotene and exhibits an energy-dissipative conformation. Using femtosecond spectroscopy, we demonstrated that the energy dissipation is achieved via direct energy transfer from a Chl-a Q(y) state to the beta-carotene S-1 state. We did not detect any cation of beta-carotene that would accompany Chl-a quenching. These results provide proof of principle that this quenching mechanism operates in the LHC superfamily and also shed light on the photoprotective role of Hlips and the evolution of LHC antennae.
Pracoviště	Mikrobiologický ústav
Kontakt	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Rok sběru	2016

Počet záznamů: 1