HP1 beta-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs

Stixová, Lenka; Sehnalová, Petra; Legartová, Soňa; Suchánková, Jana; Hrušková, Tereza; Sorokin, D.V.; Matula, Pa.; Raška, I.; Kovařík, Aleš; Fulneček, Jaroslav; Bártová, Eva

doi:https://dx.doi.org/10.1186/1756-8935-7-39

Počet záznamů: 1

HP1 beta-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs

1.

SYSNO ASEP	0441300
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	HP1 beta-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs
Tvůrce(i)	Stixová, Lenka (BFU-R)_{RID, ORCID} Sehnalová, Petra (BFU-R) Legartová, Soňa (BFU-R)_ORCID Suchánková, Jana (BFU-R) Hrušková, Tereza (BFU-R) Sorokin, D.V. (CZ) Matula, Pa. (CZ) Raška, I. (CZ) Kovařík, Aleš (BFU-R)_{RID, ORCID} Fulneček, Jaroslav (BFU-R) Bártová, Eva (BFU-R)_ORCID
Celkový počet autorů	11
Zdroj.dok.	Epigenetics & Chromatin - ISSN 1756-8935 Roč. 7, DEC2014 (2014), s. 1-17
Poč.str.	17 s.
Forma vydání	Online - E
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	DNA-damage response ; DNA repair ; Irradiation
Vědní obor RIV	BO - Biofyzika
CEP	GAP302/10/1022 GA ČR - Grantová agentura ČR
	GBP302/12/G157 GA ČR - Grantová agentura ČR
	GA13-07822S GA ČR - Grantová agentura ČR
Institucionální podpora	BFU-R - RVO:68081707
UT WOS	000347931500001
DOI	https://doi.org/10.1186/1756-8935-7-39
Anotace	Background: The repair of spontaneous and induced DNA lesions is a multistep process. Depending on the type of injury, damaged DNA is recognized by many proteins specifically involved in distinct DNA repair pathways. Results: We analyzed the DNA-damage response after ultraviolet A (UVA) and. irradiation of mouse embryonic fibroblasts and focused on upstream binding factor 1 (UBF1), a key protein in the regulation of ribosomal gene transcription. We found that UBF1, but not nucleolar proteins RPA194, TCOF, or fibrillarin, was recruited to UVA-irradiated chromatin concurrently with an increase in heterochromatin protein 1 beta (HP1 beta) level. Moreover, Forster Resonance Energy Transfer (FRET) confirmed interaction between UBF1 and HP1 beta that was dependent on a functional chromo shadow domain of HP1 beta. Thus, overexpression of HP1 beta with a deleted chromo shadow domain had a dominant-negative effect on UBF1 recruitment to UVA-damaged chromatin.
Pracoviště	Biofyzikální ústav
Kontakt	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Rok sběru	2015

Počet záznamů: 1