LITTER EFFECT IN MOUSE PHENOTYPIC STUDIES

0441201 - ÚMG 2015 RIV PT eng C - Konferenční příspěvek (zahraniční konf.)
Šimeček, Petr - Dzúr-Gejdošová, Mária - Chvátalová, I. - Forejt, Jiří
LITTER EFFECT IN MOUSE PHENOTYPIC STUDIES.
BIOINFORMATICS 2011, Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms. SETUBAL, PORTUGAL: SCITEPRESS, AV D MANUELL, 27A 2 ESQ, SETUBAL, 2910-595, PORTUGAL, 2011, s. 238-243. ISBN 978-989-8425-36-2.
[BIOINFORMATICS 2011, Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms. Setubal (PT), 26.01.2011-29.01.2011]
Institucionální podpora: RVO:68378050
Klíčová slova: Litter effect * Mixed-effect models * Phenome databases * Mouse genetics
Kód oboru RIV: EB - Genetika a molekulární biologie

The laboratory mouse is the most common mammalian model organism for research of the human body functions and disorders. For experimental purposes mice selected from inbred strains, developed by many generations of brother-sister crosses, are usually used. Individual mice of a given inbred strain are therefore considered genetically identical. However, our preliminary observations suggest that for a number of phenotypic traits mice originating from the same litter are significantly more similar than mice coming from different litters of the same inbred strain. We estimated the size of this litter effect for a number of traits in several phenotypic studies. By means of simulation we showed that ignoring the litter effect may result in several fold higher false positive rate and severe underestimation of minimal sample size.
Trvalý link: http://hdl.handle.net/11104/0244271