Počet záznamů: 1
Phytoplasmas and phytoplasma disease management: how to reduce their economic impact
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SYSNO ASEP 0439207 Druh ASEP M - Kapitola v monografii Zařazení RIV C - Kapitola v knize Název Phytoplasma infections in Rhododendron hybridum Tvůrce(i) Přibylová, Jaroslava (BC-A) RID, ORCID
Špak, Josef (BC-A) RID, ORCID
Fránová, Jana (BC-A) RID, ORCIDZdroj.dok. Phytoplasmas and phytoplasma disease management: how to reduce their economic impact, The phytoplasmas and phytoplasma vectors in COST FA0807 Countries. - Bologna : IPWG - International Phytoplasmologist Working Group, 2014 / Bertaccini Assunta - ISBN 978-88-909922-0-9 Rozsah stran s. 137-142 Poč.str. 6 s. Poč.str.knihy 287 Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. IT - Itálie Klíč. slova Electron microscopy ; PCR ; phytoplasma ; sequencing Vědní obor RIV EE - Mikrobiologie, virologie CEP LD12074 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BC-A - RVO:60077344 Anotace The survey of the health status of azaleas and rhododendrons on production areas in the Czech Republic started in 2006. Symptoms of unknown aetiology on Rhododendron hybridum cultivar Cunningham’s White were observed in 2010. The infected plant had malformed leaves, with irregular shaped edges, mosaic, leaf tip necrosis and multiple axillary shoots with smaller leaves. Transmission electron microscopy showed phytoplasma-like bodies in phloem cells of the symptomatic plant. Phytoplasma presence was confirmed by polymerase chain reaction using phytoplasma-specific, universal and group specific primer pairs. Restriction fragment length polymorphism analysis of 16S rDNA enabled classification of the detected phytoplasma in the aster yellows subgroup 16SrI-C. Sequence analysis of the 16S-23S ribosomal operon of the amplified phytoplasma from the infected rhododendron plant (1,724 bp) confirmed the closest relationship with the Czech Echinacea purpurea phyllody phytoplasma. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2015
Počet záznamů: 1