- Mutation in the Drosophila melanogaster adenosine receptor gene selec…
Počet záznamů: 1  

Mutation in the Drosophila melanogaster adenosine receptor gene selectively decreases the mosaic hyperplastic epithelial outgrowth rates in wts or dco heterozygous flies

  1. 1.
    SYSNO ASEP0438115
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevMutation in the Drosophila melanogaster adenosine receptor gene selectively decreases the mosaic hyperplastic epithelial outgrowth rates in wts or dco heterozygous flies
    Tvůrce(i) Sidorov, Roman (BC-A) RID, ORCID
    Kučerová, Lucie (BC-A) RID, ORCID
    Kiss, I. (HU)
    Žurovec, Michal (BC-A) RID, ORCID
    Celkový počet autorů4
    Zdroj.dok.Purinergic Signalling - ISSN 1573-9538
    Roč. 11, č. 1 (2015), s. 95-105
    Poč.str.11 s.
    Jazyk dok.eng - angličtina
    Země vyd.NL - Nizozemsko
    Klíč. slovacell competition ; Adgf-A ; Ent2
    Vědní obor RIVEB - Genetika a molekulární biologie
    CEPGA14-27816S GA ČR - Grantová agentura ČR
    Institucionální podporaBC-A - RVO:60077344
    UT WOS000350883100006
    EID SCOPUS84925535445
    DOI https://doi.org/10.1007/s11302-014-9435-2
    AnotaceAdenosine (Ado) is a purine nucleoside that modulates many physiological processes in the body. It plays a prominent role as a paracrine signal of metabolic imbalance within tissues and serves as a signaling mechanism to coordinate tissue activity. Ado exerts a broad range of cytoprotective, growth-promoting, and immunosuppressive effects and was observed at a high concentration in a number of human tumors, it was therefore suggested to be an important factor regulating tumor growth. We developed a method for the measurement of the Ado effect on tumor growth in/Drosophila melanogaster/using the modification of somatic mutation and recombination test (SMART). In this report, we examined the effect of three/Drosophila/genes involved in Ado signaling on the incidence of somatic mosaic clones, including adenosine receptor (/AdoR/), adenosine transporter (/Ent2/), and adenosine deaminase/Adgf-A/. We show that genetic manipulations with these genes do not affect control clones, but cause dramatic changes in the frequency of hyperplastic clones established by the loss of heterozygosity (LOH) of the/warts/(/wts/) tumor suppressor gene/(wts/homolog in humans is called/LATS1/and its down-regulation has been reported in a number of tumors). The loss of/AdoR/function also decreases the frequency of/dco/tumor clones (human homolog of/dco/is called CK1δ). Our data show that adenosine and/warts/signaling pathways interact and adenosine signaling plays an important role in growth and survival of homozygous/wts^- /cells.
    PracovištěBiologické centrum (od r. 2006)
    KontaktDana Hypšová, eje@eje.cz, Tel.: 387 775 214
    Rok sběru2016
    Elektronická adresahttp://link.springer.com/article/10.1007%2Fs11302-014-9435-2
Počet záznamů: 1  

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