Počet záznamů: 1
Time-Resolved Fluorescence in Lipid Bilayers: Selected Applications and Advantages over Steady State
- 1.
SYSNO ASEP 0436040 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Time-Resolved Fluorescence in Lipid Bilayers: Selected Applications and Advantages over Steady State Tvůrce(i) Amaro, Mariana Manuela (UFCH-W) RID, ORCID
Šachl, Radek (UFCH-W) RID, ORCID
Jurkiewicz, Piotr (UFCH-W) RID, ORCID
Coutinho, A. (PT)
Prieto, M. (PT)
Hof, Martin (UFCH-W) RID, ORCIDZdroj.dok. Biophysical Journal. - : Cell Press - ISSN 0006-3495
Roč. 107, č. 12 (2014), s. 2751-2760Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova nanoparticles ; reactivity ; energetics Vědní obor RIV CF - Fyzikální chemie a teoretická chemie CEP GC14-03141J GA ČR - Grantová agentura ČR LH13259 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UFCH-W - RVO:61388955 UT WOS 000346434200007 EID SCOPUS 84920127267 DOI https://doi.org/10.1016/j.bpj.2014.10.058 Anotace Fluorescence methods are versatile tools for obtaining dynamic and topological information about biomembranes because the molecular interactions taking place in lipid membranes frequently occur on the same timescale as fluorescence emission. The fluorescence intensity decay, in particular, is a powerful reporter of the molecular environment of a fluorophore. The fluorescence lifetime can be sensitive to the local polarity, hydration, viscosity, and/or presence of fluorescence quenchers/ energy acceptors within several nanometers of the vicinity of a fluorophore. Illustrative examples of how time-resolved fluorescence measurements can provide more valuable and detailed information about a system than the time-integrated (steady-state) approach will be presented in this review: 1), determination of membrane polarity and mobility using time-dependent spectral shifts; 2), identification of submicroscopic domains by fluorescence lifetime imaging microscopy; 3), elucidation of membrane leakage mechanisms from dye self-quenching assays; and 4), evaluation of nanodomain sizes by time-resolved Fo¨ rster resonance energy transfer measurements. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2015
Počet záznamů: 1