Počet záznamů: 1
Visualizing Stable Features in Live Cell Nucleus for Evaluation of the Cell Global Motion Compensation
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SYSNO ASEP 0435958 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Visualizing Stable Features in Live Cell Nucleus for Evaluation of the Cell Global Motion Compensation Tvůrce(i) Sorokin, D.V. (CZ)
Suchánková, Jana (BFU-R)
Bártová, Eva (BFU-R) ORCID
Matula, P. (CZ)Celkový počet autorů 4 Zdroj.dok. Folia Biologica. - : Univerzita Karlova v Praze - ISSN 0015-5500
Roč. 60, č. 1 (2014), s. 45-49Poč.str. 5 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova cell global motion compensation ; UV laser bleaching ; image registration Vědní obor RIV BO - Biofyzika CEP GBP302/12/G157 GA ČR - Grantová agentura ČR EE2.3.30.0030 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BFU-R - RVO:68081707 UT WOS 000343275800007 Anotace The compensation of cell motion is an important step in single-particle tracking analysis of live cells. This step is required in most of the cases, since the movement of subcellular foci is superimposed by the movement and deformation of the cell, while only the local motion of the foci is important to be analysed. The cell motion and deformation compensation is usually performed by means of image registration. There are a number of approaches with different models and properties presented in the literature that perform cell image registration. However, the evaluation of the registration approach quality on real data is a tricky problem due to the fact that some stable features in the images with a priori no local motion are needed. In this paper we propose a methodology for creating live cell nuclei image sequences with stable features imposed. The features are defined using the regions of fluorescence bleaching invoked by the UV laser. Data with different deformations are acquired and can be used for evaluation of the cell image registration methods. Along with that, we describe an image analysis technique and a metric that can characterize the quality of the method quantitatively. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2015
Počet záznamů: 1