Počet záznamů: 1
Ménage-a-trois: The amoeba Nuclearia sp. from Lake Zurich with its ecto- and endosymbiotic bacteria
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SYSNO ASEP 0434504 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Ménage-a-trois: The amoeba Nuclearia sp. from Lake Zurich with its ecto- and endosymbiotic bacteria Tvůrce(i) Dirren, S. (CH)
Salcher, Michaela M. (BC-A) RID, ORCID
Blom, J. F. (CH)
Schweikert, M. (DE)
Posch, T. (CH)Zdroj.dok. Protist. - : Elsevier - ISSN 1434-4610
Roč. 165, č. 5 (2014), s. 745-758Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova bacteria-protist symbioses ; ectosymbionts ; endosymbionts ; feeding ; Nucleariidae ; Paucibacter toxinivorans Vědní obor RIV DA - Hydrologie a limnologie Institucionální podpora BC-A - RVO:60077344 UT WOS 000344236600013 EID SCOPUS 84908346334 DOI https://doi.org/10.1016/j.protis.2014.08.004 Anotace We present a fascinating triad relationship between a eukaryotic amoeba and its two bacterial symbionts. The morphological characteristics of the amoeba allowed for a confident assignment to the genus Nuclearia (Opisthokonta, Nucleariidae), but species identification resulted in an ambiguous result. Sequence analysis indicated an affiliation to the species N. thermophila, however, several morphological features contradict the original description. Amoebal isolates were cultured for several years with their preferred food source, the microcystin-producing harmful cyanobacterium Planktothrix rubescens. Symbioses of the amoeba with ecto- and endosymbiotic bacteria were maintained over this period. Several thousand cells of the ectosymbiont are regularly arranged inside a layer of extracellular polymeric substances produced by the amoeba. The ectosymbiont was identified as Paucibacter toxinivorans (Betaproteobacteria), which was originally isolated by enrichment with microcystins. We found indications that our isolated ectosymbiont indeed contributed to toxin-degradation. The endosymbiont (Gammaproteobacteria, 15-20 bacteria per amoeba) is enclosed in symbiosomes inside the host cytoplasm and represents probably an obligate symbiont. We propose the name “Candidatus Endonucleariobacter rarus” for this bacterium that was neither found free-living nor in a symbiotic association. Nucleariidae are uniquely suited model organisms to study the basic principles of symbioses between opisthokonts and prokaryotes. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2015
Počet záznamů: 1