Počet záznamů: 1
Proteomic analysis of changes in the protein composition of MCF-7 human breast cancer cells induced by all-trans retinoic acid, 9-cis retinoic acid, and their combination
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SYSNO ASEP 0434131 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Proteomic analysis of changes in the protein composition of MCF-7 human breast cancer cells induced by all-trans retinoic acid, 9-cis retinoic acid, and their combination Tvůrce(i) Flodrová, Dana (UIACH-O) ORCID
Benkovská, Dagmar (UIACH-O)
Macejová, D. (SK)
Bialesova, L. (SK)
Hunakova, L. (SK)
Brtko, J. (SK)
Bobálová, Janette (UIACH-O) RID, ORCIDCelkový počet autorů 7 Zdroj.dok. Toxicology Letters. - : Elsevier - ISSN 0378-4274
Roč. 232, č. 1 (2015), s. 226-232Poč.str. 7 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. IE - Irsko Klíč. slova retinoic acid ; polyacrylamide gel electrophoresis ; MALDI TOF MS Vědní obor RIV CB - Analytická chemie, separace CEP 7AMB12SK151 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UIACH-O - RVO:68081715 UT WOS 000346175100026 DOI https://doi.org/10.1016/j.toxlet.2014.09.030 Anotace Retinoic acid (all-trans and 9-cis) isomers represent important therapeutic agents for many types of cancers, including human breast cancer. Changes in protein composition of the MCF-7 human breast cancer cells were induced by all-trans retinoic acid, 9-cis retinoic acid, and their combination and subsequently proteomic strategies based on bottom-up method were applied. Proposed approach was used for the analysis of proteins extracted from MCF-7 human breast cancer cell line utilizing a commercially manufactured kit RIPA and separated on two dimensional (2D) sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) after treatment with both retinoic acid isomers. We found significant differences in occurrence of proteins probably affecting the cell migration process in tumour cells. Heat shock protein 27, ribonucleoprotein SmD3, and cofilin-1 were significantly upregulated after treatment with combination of individual retinoic acid isomers. On the other hand, AP-5 complex subunit beta-1 shows the different response. Thus, the results might help to find the answer to important medical questions on (i) the identification of signaling pathways affected by retinoic acid isomers or (ii) how the observed proteomic pattern might reflect the effectiveness of retinoic acids treatment. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2015
Počet záznamů: 1