Počet záznamů: 1
PLANT CHEMICAL GENOMICS: METHODS AND PROTOCOLS
- 1.
SYSNO ASEP 0430550 Druh ASEP M - Kapitola v monografii Zařazení RIV C - Kapitola v knize Název Analyzing the In Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters Tvůrce(i) Simon, Sibu (UEB-Q) ORCID
Skůpa, Petr (UEB-Q) RID
Dobrev, Petre (UEB-Q) RID, ORCID
Petrášek, Jan (UEB-Q) RID, ORCID
Zažímalová, Eva (UEB-Q) RID, ORCID
Friml, J. (BE)Zdroj.dok. PLANT CHEMICAL GENOMICS: METHODS AND PROTOCOLS. - Totowa : Humana Press, 2014 / Hicks G.R. ; Robert S. - ISSN 1064-3745 - ISBN 978-1-62703-591-0 Rozsah stran s. 255-264 Poč.str. 10 s. Poč.výt. 2000 Poč.str.knihy 269 Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Auxin ; Auxin metabolism ; BY-2 tobacco cells Vědní obor RIV EB - Genetika a molekulární biologie CEP GAP305/11/0797 GA ČR - Grantová agentura ČR Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000328941100024 DOI 10.1007/978-1-62703-592-7_23 Anotace Exogenous application of biologically important molecules for plant growth promotion and/or regulation is very common both in plant research and horticulture. Plant hormones such as auxins and cytokinins are classes of compounds which are often applied exogenously. Nevertheless, plants possess a well-established machinery to regulate the active pool of exogenously applied compounds by converting them to metabolites and conjugates. Consequently, it is often very useful to know the in vivo status of applied compounds to connect them with some of the regulatory events in plant developmental processes. The in vivo status of applied compounds can be measured by incubating plants with radiolabeled compounds, followed by extraction, purification, and HPLC metabolic profiling of plant extracts. Recently we have used this method to characterize the intracellularly localized PIN protein, PIN5. Here we explain the method in detail, with a focus on general application. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2015
Počet záznamů: 1