Počet záznamů: 1
Evaluation of antibacterial properties of novel phthalocyanines against Escherichia coli - comparison of analytical methods
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SYSNO ASEP 0430204 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Evaluation of antibacterial properties of novel phthalocyanines against Escherichia coli - comparison of analytical methods Tvůrce(i) Mikula, Přemysl (BU-J)
Kalhotka, L. (CZ)
Jančula, Daniel (BU-J) RID
Zezulka, Štěpán (BU-J) ORCID
Kořínková, R. (CZ)
Černý, J. (CZ)
Maršálek, Blahoslav (BU-J) ORCID, SAI
Toman, Petr (UMCH-V) RID, ORCIDCelkový počet autorů 8 Zdroj.dok. Journal of Photochemistry and Photobiology. B - Biology Section. - : Elsevier - ISSN 1011-1344
Roč. 138, Sep 2014 (2014), s. 230-239Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova phthalocyanines ; photosensitizers ; antibacterial effect Vědní obor RIV EF - Botanika Vědní obor RIV – spolupráce Ústav makromolekulární chemie - Makromolekulární chemie CEP TA01010356 GA TA ČR - Technologická agentura ČR FR-TI3/196 GA MPO - Ministerstvo průmyslu a obchodu GAP205/10/2280 GA ČR - Grantová agentura ČR Institucionální podpora BU-J - RVO:67985939 ; UMCH-V - RVO:61389013 UT WOS 000340987500027 EID SCOPUS 8490374045 DOI 10.1016/j.jphotobiol.2014.04.014 Anotace We analyzed antibacterial effects of several novel phthalocyanines against E. coli and evaluated the suitability of flow cytometry for the detection of antibacterial effects of phthalocyanines in comparison with routinely used cultivation. After 3 hours of exposure under cool white light eight cationic phthalocyanines showed very high antibacterial activity in the concentration of 2.00 mg L-1 and four of them were even efficient in the concentration of 0.20 mg L-1. Antibacterial activity of neutral and anionic compounds was considerably lower or even negligible. Agglomeration of cells observed in suspensions treated with a higher concentration of certain cationic phthalocyanines affected cytometric measurements of total cell counts, therefore this parameter seems not to be fully valid in the evaluation of phthalocyanine antibacterial activity. On the other hand, cytometric measurement of cell membrane integrity appears to be a suitable and sensitive parameter. Pracoviště Botanický ústav Kontakt Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8 Rok sběru 2015
Počet záznamů: 1