Počet záznamů: 1
Translation initiation factors eIF3 and HCR1 control translation termination and stop codon read-through in yeast cells
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SYSNO ASEP 0426120 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Translation initiation factors eIF3 and HCR1 control translation termination and stop codon read-through in yeast cells Tvůrce(i) Beznosková, P. (CZ)
Cuchalová, Lucie (UMCH-V) RID
Wagner, S. (CZ)
Shoemaker, Ch. J. (US)
Gunišová, S. (CZ)
von der Haar, T. (GB)
Valášek, L. S. (CZ)Zdroj.dok. PLoS Genetics. - : Public Library of Science - ISSN 1553-7404
Roč. 9, č. 11 (2013), e1003962_1-e1003962_17Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova translation initiation ; translation termination ; eIF3 Vědní obor RIV CD - Makromolekulární chemie Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000330369000042 EID SCOPUS 84888230681 DOI 10.1371/journal.pgen.1003962 Anotace Translation is divided into initiation, elongation, termination and ribosome recycling. Earlier work implicated several eukaryotic initiation factors (eIFs) in ribosomal recycling in vitro. Here, we uncover roles for HCR1 and eIF3 in translation termination in vivo. A substantial proportion of eIF3, HCR1 and eukaryotic release factor 3 (eRF3) but not eIF5 (a well-defined “initiation-specific” binding partner of eIF3) specifically co-sediments with 80S couples isolated from RNase-treated heavy polysomes in an eRF1-dependent manner, indicating the presence of eIF3 and HCR1 on terminating ribosomes. eIF3 and HCR1 also occur in ribosome- and RNA-free complexes with both eRFs and the recycling factor ABCE1/RLI1. Several eIF3 mutations reduce rates of stop codon read-through and genetically interact with mutant eRFs. In contrast, a slow growing deletion of hcr1 increases read-through and accumulates eRF3 in heavy polysomes in a manner suppressible by overexpressed ABCE1/RLI1. Based on these and other findings we propose that upon stop codon recognition, HCR1 promotes eRF3•GDP ejection from the post-termination complexes to allow binding of its interacting partner ABCE1/RLI1. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2014
Počet záznamů: 1