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Plant Lipid Signaling Protocols
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SYSNO ASEP 0423956 Druh ASEP M - Kapitola v monografii Zařazení RIV C - Kapitola v knize Název Assaying nonspecific phospholipase C activity Tvůrce(i) Pejchar, Přemysl (UEB-Q) RID, ORCID
Günther, F.E.S. (DE)
Martinec, Jan (UEB-Q) RID, ORCIDZdroj.dok. Plant Lipid Signaling Protocols, Vol. 1009. - New York City : Humana Press, 2013 / Munnik T. ; Heilmann I. - ISSN 1064-3745 - ISBN 978-1-62703-400-5 Rozsah stran s. 193-203 Poč.str. 11 s. Poč.výt. 5000 Poč.str.knihy 305 Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Bodipy ; Diacylglycerol ; Nonspecific phospholipase C Vědní obor RIV ED - Fyziologie CEP GAP501/12/1942 GA ČR - Grantová agentura ČR GPP501/12/P950 GA ČR - Grantová agentura ČR CEZ AV0Z50380511 - UEB-Q (2005-2011) EID SCOPUS 84883229342 DOI 10.1007/978-1-62703-401-2_18 Anotace Plant nonspecific phospholipase C (NPC) is a recently described enzyme which plays a role in membrane rearrangement during phosphate starvation. It is also involved in responses of plants to brassinolide, abscisic acid (ABA), elicitors, and salt. The NPC activity is decreased in cells treated with aluminum. In the case of salt stress, the molecular mechanism of NPC action is based on accumulation of diacylglycerol (DAG) by hydrolysis of phospholipids and conversion of DAG, the product of NPC activity, to phosphatidic acid (PA) that participates in ABA signaling pathways. Here we describe a step-by-step protocol, which can be used to determine in situ or in vitro NPC activity. Determination is based on quantification of fluorescently labeled DAG as a product of cleavage of the fluorescently labeled substrate lipid, phosphatidylcholine. High-performance thin-layer chromatography is used for separation of fluorescent DAG. The spot is visualized with a laser scanner and the relative amounts of fluorescent DAG are quantified using imaging software. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2014
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