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Overexpressed TPX2 causes ectopic formation of microtubular arrays in the nuclei of acentrosomal plant cells
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SYSNO ASEP 0421945 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Overexpressed TPX2 causes ectopic formation of microtubular arrays in the nuclei of acentrosomal plant cells Tvůrce(i) Petrovská, Beáta (UEB-Q) RID, ORCID
Jeřábková, Hana (UEB-Q) RID, ORCID
Kohoutová, Lucie (MBU-M) RID
Cenklová, Věra (UEB-Q)
Pochylová, Žaneta (UEB-Q)
Gelová, Zuzana (UEB-Q)
Kočárová, Gabriela (MBU-M)
Váchová, Lenka (UEB-Q)
Kurejová, Michaela (UEB-Q)
Tomaštíková, Eva (UEB-Q) RID, ORCID
Binarová, Pavla (MBU-M) RID, ORCIDZdroj.dok. Journal of Experimental Botany. - : Oxford University Press - ISSN 0022-0957
Roč. 64, č. 14 (2013), s. 4575-4587Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Arabidopsis thaliana ; AtTPX2 ; Aurora kinase Vědní obor RIV EB - Genetika a molekulární biologie CEP GAP501/12/2333 GA ČR - Grantová agentura ČR GBP501/12/G090 GA ČR - Grantová agentura ČR GP204/09/P155 GA ČR - Grantová agentura ČR GA204/07/1169 GA ČR - Grantová agentura ČR Institucionální podpora UEB-Q - RVO:61389030 ; MBU-M - RVO:61388971 UT WOS 000326724700034 DOI 10.1093/jxb/ert271 Anotace TPX2 performs multiple roles in microtubule organization. Previously, it was shown that plant AtTPX2 binds AtAurora1 kinase and colocalizes with microtubules in a cell cycle-specific manner. To elucidate the function of TPX2 further, this work analysed Arabidopsis cells overexpressing AtTPX2-GFP. Distinct arrays of bundled microtubules, decorated with AtTPX2-GFP, were formed in the vicinity of the nuclear envelope and in the nuclei of overexpressing cells. The microtubular arrays showed reduced sensitivity to anti-microtubular drugs. TPX2-mediated formation of nuclear/perinuclear microtubular arrays was not specific for the transition to mitosis and occurred independently of Aurora kinase. The fibres were not observed in cells with detectable programmed cell death and, in this respect, they differed from TPX2-dependent microtubular assemblies functioning in mammalian apoptosis. Colocalization and co-purification data confirmed the interaction of importin with AtTPX2-GFP. In cells with nuclear foci of overexpressed AtTPX2-GFP, strong nuclear signals for Ran and importin diminished when microtubular arrays were assembled. This observation suggests that TPX2-mediated microtubule formation might be triggered by a Ran cycle. Collectively, the data suggest that in the acentrosomal plant cell, in conjunction with importin, overexpressed AtTPX2 reinforces microtubule formation in the vicinity of chromatin and the nuclear envelope. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2014
Počet záznamů: 1