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Microfluidic systems for optical sorting
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SYSNO ASEP 0388103 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Microfluidic systems for optical sorting Author(s) Ježek, Jan (UPT-D) RID, ORCID, SAI
Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
Šerý, Mojmír (UPT-D) RID, SAI
Kaňka, Jan (UPT-D) RID, SAI
Samek, Ota (UPT-D) RID, ORCID, SAI
Bernatová, Silvie (UPT-D) RID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCIDNumber of authors 7 Source Title CPS 2012. 18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics (Proceedings of SPIE Vol.8697). - Bellingham : SPIE, 2012 - ISBN 978-0-8194-9481-8 Pages 86970w: 1-9 Number of pages 9 s. Publication form Print - P Action CPS 2012. Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics /18./ Event date 03.09.2012-07.09.2012 VEvent location Ostravice Country CZ - Czech Republic Event type EUR Language eng - English Country US - United States Keywords microfluidic ; cell sorting ; optical tweezers ; Raman spectroscopy Subject RIV BH - Optics, Masers, Lasers R&D Projects FR-TI1/433 GA MPO - Ministry of Industry and Trade (MPO) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GAP205/11/1687 GA ČR - Czech Science Foundation (CSF) Institutional support UPT-D - RVO:68081731 UT WOS 000319864100032 DOI https://doi.org/10.1117/12.2008649 Annotation We have constructed a device for active optical manipulation and Raman spectral analysis in a microfluidic channel for efficient, nondestructive and contactless sorting of biological samples based on the Raman spectroscopic characteristics of living cells. In our previous work, we have linked such Raman spectral characteristics of microalgal lipid bodies with the unsaturation or carotene concentration via a calibration curve. As the sorting platform we have used a combination of fast galvano-optic laser steering system and specially designed microfluidic chips. We used X shaped channels with two input and output ports, and also several differently shaped variants. The steerable trapping laser beam was designed to move the cell to the specified locations and confine the cell for the time period needed for the analysis. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2013
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