Počet záznamů: 1
The analysis of PS II photochemical activity using single and multi-turnover excitations
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SYSNO ASEP 0387345 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The analysis of PS II photochemical activity using single and multi-turnover excitations Tvůrce(i) Vredenberg, W. (NL)
Durchan, M. (CZ)
Prášil, Ondřej (MBU-M) RID, ORCIDZdroj.dok. Journal of Photochemistry and Photobiology. B - Biology Section. - : Elsevier - ISSN 1011-1344
Roč. 107, FEB 6 2012 (2012), s. 45-54Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Photochemical activity ; Photosystem II ; Chlorophyll a fluorescence Vědní obor RIV EE - Mikrobiologie, virologie CEP GA206/08/1683 GA ČR - Grantová agentura ČR ED2.1.00/03.0110 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy 7E09099 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000300477100007 DOI https://doi.org/10.1016/j.jphotobiol.2011.11.009 Anotace Paper describes chlorophyll a fluorescence measurements in algal cells, and intact plant leaves and isolated chloroplasts. It focuses on amplitude and 10 mu-resolved kinetics of variable fluorescence responses upon excitation with fluorescence-saturating pulses (SP) and with 25 mu s saturating single turnover flashes (STF) which are exposed before, during and after a 100 s actinic illumination (AL) of low and high intensity. In addition to the amply documented suppression of the maximal variable fluorescence from F-m to F'(m), the relative proportion of the distinguished O-J-, J-I- and I-P-phases of an SP-induced response is shown to be distinctly different in dark- and light-adapted leaves. The O-J-phase in the 0.01-1 ms time range is much less sensitive to light adaptation than the other phases in the 1-200 ms range. In algae and chloroplasts, the amplitude F-m(ST) of the STF-induced response is hardly affected by a shift from the dark- to the light-activated steady state. The results support the hypothesis that the maximal variable fluorescence F, induced by a multiple-turnover, fluorescence-saturating pulse (SP), is associated with the release of photochemical and photoelectrochemical quenching Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2013
Počet záznamů: 1