Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA

0383454 - BFÚ 2013 RIV NL eng J - Článek v odborném periodiku
Bartošík, Martin - Fojta, Miroslav - Paleček, Emil
Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA.
Electrochimica acta. Roč. 78, SEP (2012), s. 75-81. ISSN 0013-4686. E-ISSN 1873-3859
Grant CEP: GA ČR(CZ) GAP301/11/2055; GA MŠMT(CZ) ME09038
Výzkumný záměr: CEZ:AV0Z50040702
Klíčová slova: DNA methylation * 5-methylcytosine analysis * sodium bisulfite
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 3.777, rok: 2012

Using DNA bisulfite treatment, reducible cytosines were transformed into nonreducible uracil residues, strongly decreasing square wave voltammetric C reduction peaks of unmethylated DNA. On the other hand, 5-methylcytosines were not transformed and the reduction peaks of methylated DNA remained relatively unchanged. By combining DNA bisulfite treatment with square wave voltammetry, DNA methylation can be determined quantitatively at nanomolar and subnanomolar ODN concentrations.
Trvalý link: http://hdl.handle.net/11104/0213388