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Magnetic bead cellulose as a suitable support for immobilization of .alpha.-chymotrypsin
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SYSNO ASEP 0382141 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Magnetic bead cellulose as a suitable support for immobilization of .alpha.-chymotrypsin Tvůrce(i) Přikryl, P. (CZ)
Lenfeld, Jiří (UMCH-V) RID
Horák, Daniel (UMCH-V) RID, ORCID
Tichá, M. (CZ)
Kučerová, Z. (CZ)Zdroj.dok. Applied Biochemistry and Biotechnology. - : Springer - ISSN 0273-2289
Roč. 168, č. 2 (2012), s. 295-305Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova enzyme immobilization ; human gastric juice ; immobilized α-chymotrypsin Vědní obor RIV CB - Analytická chemie, separace CEP GA203/09/0857 GA ČR - Grantová agentura ČR CEZ AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000309224300007 DOI https://doi.org/10.1007/s12010-012-9772-y Anotace Magnetic bead cellulose was prepared by a suspension method from the mixture of viscose and magnetite using thermal sol–gel transition and regeneration of cellulose. The prepared magnetic particles after their activation with divinyl sulfone were shown to be suitable magnetic carrier for immobilization of α-chymotrypsin and for its application in proteomic studies. The specific activity of the immobilized proteinase was high; its activity did not change in the course of storage. The following properties of the immobilized proteinase were compared with those of the soluble enzyme: pH and temperature dependence of the activity, self-cleavage activity, and possibility of repeated use. α-Chymotrypsin immobilized to magnetic bead cellulose was used for the proteolytic digestion of porcine pepsin A and human gastric juice and a possibility of direct use of enzyme reaction products for matrix-assisted laser desorption/ionization time of flight mass spectrometry analysis was shown. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2013
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