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The transcription factor AtMYB75/PAP1 regulates the expression of flavonoid biosynthesis genes in transgenic hop (Humulus lupulus L.)
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SYSNO ASEP 0381542 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název The transcription factor AtMYB75/PAP1 regulates the expression of flavonoid biosynthesis genes in transgenic hop (Humulus lupulus L.) Tvůrce(i) Gatica-Arias, A. (DE)
Farag, M.A. (DE)
Häntzschel, K.R. (DE)
Matoušek, Jaroslav (BC-A) RID, ORCID
Weber, G. (DE)Celkový počet autorů 5 Zdroj.dok. Brewing Science - ISSN 1866-5195
Roč. 65, 7-8 (2012), s. 103-111Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova metabolic engineering ; Humulus lupulus L. ; transcription factors ; flavonoid biosynthesis Vědní obor RIV EB - Genetika a molekulární biologie CEP GA521/08/0740 GA ČR - Grantová agentura ČR Institucionální podpora BC-A - RVO:60077344 CEZ AV0Z50510513 - UMBR-M, BC-A (2005-2011) Anotace Metabolic engineering of the flavonoid pathway may be used for modifying nutritional, pharmaceutical and ornamental properties of plants. The PAP1 (AtMYB75) is a R2R3 MYB family member and stimulates the expression of flavonoid pathway genes in Arabidopsis thaliana . Previously, PAP1 was introduced into Humulus lupulus plants by Agrobacterium-mediated genetic transformation. Employing quantitative PCR, we show that each transgenic plant harbors one copy of PAP1. The expression of the structural genes CHS_H1, CHI, and F3'H, essential for the biosynthesis of flavonoids, was elevated in PAP1 transgenic plants compared to the wildtype hop. Chemometric methods were successfully used to discriminate between wildtype and transgenic plants expressing the transcription factor PAP1. Thus, transcription factor PAP1 activated the expression of these three genes in transgenic plants, opening the possibility for improving the content of pharmaceutically important secondary metabolites in hop. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2013
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