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Efficient expression of Human papillomavirus 16 E7 oncoprotein fused to C-terminus of Tobacco mosaic virus (TMV) coat protein using molecular chaperones in Escherichia coli
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SYSNO ASEP 0380787 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Efficient expression of Human papillomavirus 16 E7 oncoprotein fused to C-terminus of Tobacco mosaic virus (TMV) coat protein using molecular chaperones in Escherichia coli Tvůrce(i) Folwarczna, Jitka (UEB-Q)
Moravec, Tomáš (UEB-Q) RID, ORCID
Plchová, Helena (UEB-Q) RID, ORCID
Hoffmeisterová, Hana (UEB-Q) RID
Čeřovská, Noemi (UEB-Q) RID, ORCIDZdroj.dok. Protein Expression and Purification. - : Elsevier - ISSN 1046-5928
Roč. 85, č. 1 (2012), s. 152-157Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Bacterial expression ; Human papillomavirus ; E7 oncoprotein Vědní obor RIV EI - Biotechnologie a bionika CEP GA521/09/1525 GA ČR - Grantová agentura ČR GAP501/12/1761 GA ČR - Grantová agentura ČR CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000308630400021 DOI 10.1016/j.pep.2012.07.008 Anotace The Human papillomavirus 16 (HPV16) E7 oncoprotein is a promising candidate for development of anti-cancer therapeutic vaccine. We have prepared the expression construct carrying mutagenized E7 oncoprotein fused to the C-terminus of Tobacco mosaic virus (TMV) coat protein via 15 amino acids β-sheet linker. The fusion protein was expressed in Escherichia coli MC 1061 cells. We have obtained high level expression, but most of the protein remained in insoluble inclusion bodies. To increase the ratio of soluble protein various molecular chaperones (TF, DnaK–DnaJ–GrpE, GroEL–GroES) were used. The immunological reactivity of expressed recombinant protein was evaluated with anti-E7 and anti-TMV antibodies. The distribution of expressed product during ultracentrifugation on sucrose gradient was studied. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2013
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