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A major invasion of transposable elements accounts for the large size of the Blumeria graminis f.sp. tritici genome
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SYSNO ASEP 0369945 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A major invasion of transposable elements accounts for the large size of the Blumeria graminis f.sp. tritici genome Tvůrce(i) Parlange, Z. (CH)
Oberhaensli, S. (CH)
Breen, J. (CH)
Platzer, M. (DE)
Taudien, S. (DE)
Šimková, Hana (UEB-Q) RID, ORCID
Wicker, T. (CH)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Keller, B. (CH)Zdroj.dok. Functional & Integrative Genomics - ISSN 1438-793X
Roč. 11, č. 4 (2011), s. 671-677Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Blumeria graminis ; BAC library ; BAC-end sequences Vědní obor RIV EB - Genetika a molekulární biologie CEP ED0007/01/01 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000300093500014 DOI 10.1007/s10142-011-0240-5 Anotace Powdery mildew of wheat (Triticum aestivum L.) is caused by the ascomycete fungus Blumeria graminis f.sp. tritici. Genomic approaches open new ways to study the biology of this obligate biotrophic pathogen. We started the analysis of the Bg tritici genome with the low-pass sequencing of its genome using the 454 technology and the construction of the first genomic bacterial artificial chromosome (BAC) library for this fungus. High-coverage contigs were assembled with the 454 reads. They allowed the characterization of 56 transposable elements and the establishment of the Blumeria repeat database. The BAC library contains 12,288 clones with an average insert size of 115 kb, which represents a maximum of 7.5-fold genome coverage. Sequencing of the BAC ends generated 12.6 Mb of random sequence representative of the genome. Analysis of BAC-end sequences revealed a massive invasion of transposable elements accounting for at least 85% of the genome. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2012
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