Počet záznamů: 1
Development of molecular assays for the identification of the 11 Eimeria species of the domestic rabbit (Oryctolagus cuniculus)
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SYSNO ASEP 0367388 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Development of molecular assays for the identification of the 11 Eimeria species of the domestic rabbit (Oryctolagus cuniculus) Tvůrce(i) Oliveira, U. C. (BR)
Fraga, J. S. (BR)
Licois, D. (FR)
Pakandl, Michal (BC-A) RID
Gruber, A. (BR)Zdroj.dok. Veterinary Parasitology. - : Elsevier - ISSN 0304-4017
Roč. 176, 2/3 (2011), 275-280Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Eimeria ; Rabbit ; Coccidiosis ; ITS1 ; Ribosomal DNA ; PCR-based diagnosis Vědní obor RIV GJ - Choroby a škůdci zvířat, veterinární medicína CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000289221400027 DOI 10.1016/j.vetpar.2010.10.054 Anotace Coccidiosis are the major parasitic diseases in poultry and other domestic animals including the domestic rabbit (Oryctolagus cuniculus). 11 distinct Eimeria species have been identified in this host, but no PCR-based method has been developed so far for unequivocal species differentiation. In this work, we describe the development of molecular diagnostic assays that allow for the detection and discrimination of the 11 Eimeria species that infect rabbits. We determined the nucleotide sequences of the ITS1 ribosomal DNAs and designed species-specific primers for each species. We performed specificity tests using heterologous sets of primers and DNA samples, and no cross-specific bands were observed. We obtained a detection limit varying from 500 fg to 1 pg, which corresponds approximately to 0.8-1.7 sporulated oocysts, respectively. The test reported here showed good reproducibility and presented a consistent sensitivity with three different brands of amplification enzymes. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2012
Počet záznamů: 1