Počet záznamů: 1
A Genomewide Screen for Tolerance to Cationic Drugs Reveals Genes Important for Potassium Homeostasis in Saccharomyces cerevisiae
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SYSNO ASEP 0364285 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A Genomewide Screen for Tolerance to Cationic Drugs Reveals Genes Important for Potassium Homeostasis in Saccharomyces cerevisiae Tvůrce(i) Barreto, L. (ES)
Canadell, D. (ES)
Petrezsélyová, Silvia (FGU-C)
Navarrete, C. (ES)
Marešová, Lydie (FGU-C)
Peréz-Valle, J. (ES)
Herrera, R. (ES)
Olier, I. (ES)
Giraldo, J. (ES)
Sychrová, Hana (FGU-C) RID, ORCID
Yenush, L. (ES)
Ramos, J. (ES)
Ariňo, J. (ES)Zdroj.dok. Eukaryotic Cell - ISSN 1535-9778
Roč. 10, č. 9 (2011), s. 1241-1250Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Potassium homeostasis ; Saccharomyces cerevisiae ; genomewide screen Vědní obor RIV EB - Genetika a molekulární biologie CEP LC531 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy IAA500110801 GA AV ČR - Akademie věd CEZ AV0Z50110509 - FGU-C (2005-2011) UT WOS 000294453000010 DOI 10.1128/EC.05029-11 Anotace Here, we describe a genome-wide screen for mutants that present altered tolerance to hygromycin B, spermine and tetramethylammonium. Two hundred twenty six mutant strains displayed altered tolerance to all three drugs (202 hypersensitive and 24 hypertolerant) and more than 50% presented a strong or moderate growth defect at limiting potassium (1 mM). Functional groups such as protein kinases and phosphatases, intracellular trafficking, transcription or cell cycle and DNA processing were enriched. Essentially, our screen has identified a substantial number of genes which were not previously described to play a direct or indirect role in potassium homeostasis. A subset of 27 representative mutants were selected and subjected to diverse biochemical tests that, in some cases, allow us to postulate the basis for the observed phenotypes Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2012
Počet záznamů: 1