Počet záznamů: 1
Hemelipoglycoprotein from the ornate sheep tick, Dermacentor marginatus: structural and functional characterization
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SYSNO ASEP 0359405 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Hemelipoglycoprotein from the ornate sheep tick, Dermacentor marginatus: structural and functional characterization Tvůrce(i) Dupejová, J. (CZ)
Štěrba, Ján (BC-A) RID, ORCID
Vancová, Marie (BC-A) RID, ORCID
Grubhoffer, Libor (BC-A) RID, ORCIDZdroj.dok. Parasites & Vectors. - : BioMed Central - ISSN 1756-3305
Roč. 4, January (2011), s. 4Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova ORNITHODOROS PARKERI ; BOOPHILUS MICROPLUS ; MASS-SPECTROMETRY ; CATTLE TICK ; SOFT TICK ; DORIN M ; PROTEINS ; HEMOLYMPH ; VITELLOGENIN ; LIPOPROTEIN Vědní obor RIV EA - Morfologické obory a cytologie CEP LC06009 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy KJB600960906 GA AV ČR - Akademie věd CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000286388600001 DOI 10.1186/1756-3305-4-4 Anotace Results: Hemelipoglycoprotein from sheep tick Dermacentor marginatus plasma was purified in native state by immunoprecipitation and denatured using electroelution from SDS-PAGE separated plasma. The protein (290 kDa) contains two subunits with molecular weights 100 and 95 kDa. It is glycosylated by high-mannose and complex N-glycans HexNAc(2)Hex(9), HexNAc(2)Hex(10), HexNAc(4)Hex(7), and HexNAc(4)Hex(8). The purified protein is able to agglutinate red blood cells and has galactose-and mannose-binding specificity. Feeding does not increase its amounts in male plasma. The hemelipoglycoprotein was detected also in hemocytes, salivary glands, and gut. In salivary glands, the protein was present in both glycosylated and nonglycosylated forms. In hemolymph, it is present in complexes with putative fibrinogen-related proteins. This, together with its carbohydrate-binding activity, suggests its possible involvement in tick innate immunity. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2012
Počet záznamů: 1