Počet záznamů: 1
Biochemical properties of three plant nucleases with anticancer potential
- 1.
SYSNO ASEP 0357370 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Biochemical properties of three plant nucleases with anticancer potential Tvůrce(i) Podzimek, Tomáš (BC-A)
Matoušek, Jaroslav (BC-A) RID, ORCID
Lipovová, P. (CZ)
Poučková, P. (CZ)
Spiwok, V. (CZ)
Šantrůček, J. (CZ)Zdroj.dok. Plant Science. - : Elsevier - ISSN 0168-9452
Roč. 180, č. 2 (2011), s. 343-351Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. IE - Irsko Klíč. slova dsRNase activity ; N-glycosylation ; Anti-tumor effect Vědní obor RIV EB - Genetika a molekulární biologie CEP GA521/09/1214 GA ČR - Grantová agentura ČR CEZ AV0Z50510513 - UMBR-M, BC-A (2005-2011) UT WOS 000286562200022 DOI 10.1016/j.plantsci.2010.10.006 Anotace Biochemical and structural properties of three recombinant (R), highly homologous, plant bifunctional nucleases from tomato (R-TBN1), hop (R-HBN1) and Arabis brassica (R-ABN1) were determined. These nucleases cleave single- and double-stranded substrates, as well as both RNA and DNA with nearly the same efficiency. In addition, they are able to cleave several artificial substrates and highly stable viroid RNA. They also possess 3'-nucleotidase activity; therefore, they can be classified as nuclease I family members. Interestingly, poly(G) is resistant to cleavage and moreover it inhibits dsDNase, ssDNase and RNase activity of the studied nucleases. All three nucleases exhibit zinc-dependence and a strong stimulatory effect of Zn2+ for dsDNA cleavage. 3-D models, predicted on the basis of experimental structure of P1 nuclease, show nine amino acid residues responsible for interactions with zinc atoms, located in the same positions as in P1 nuclease. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2012
Počet záznamů: 1