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Removable nanocoatings for siRNA polyplexes
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SYSNO ASEP 0356597 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Removable nanocoatings for siRNA polyplexes Tvůrce(i) Kostka, Libor (UMCH-V) RID, ORCID
Koňák, Čestmír (UMCH-V) RID
Šubr, Vladimír (UMCH-V) RID, ORCID
Špírková, Milena (UMCH-V) RID, ORCID
Addadi, Y. (IL)
Neeman, M. (IL)
Lammers, T. (NL)
Ulbrich, Karel (UMCH-V) RIDZdroj.dok. Bioconjugate Chemistry. - : American Chemical Society - ISSN 1043-1802
Roč. 22, č. 2 (2011), s. 169-179Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova polyelectrolyte complexes ; siRNA ; nanocoating Vědní obor RIV CD - Makromolekulární chemie CEP IAAX00500803 GA AV ČR - Akademie věd KAN200200651 GA AV ČR - Akademie věd CEZ AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000287297000009 DOI 10.1021/bc100197e Anotace Intracellularly removable polymer nanocoatings designed for surface modification of siRNA-based polyplexes (PEC) were developed with the aim to improve properties of PEC in physiological environment. N-(2-hydroxypropyl)methacrylamide (HPMA) based copolymers containing reactive groups bound via biodegradable disulfide spacers to the polymeric backbone were synthesized and used for coating of PEC formed by the self-assembly of siRNA with the polycations polyethylene imine and poly(HPMA)-grafted poly(l-lysine) (GPL). Light scattering analysis showed the attachment of, on average, 28 polymer molecules to the surface of the polyplexes forming a 5-nm-thick hydrophilic stealth coating. Coating of the siRNA/GPL PEC substantially improved their stability in physiological solutions and an initial proof-of-principle for time- and concentration-dependent specific gene silencing was obtained. It was demonstrated that siRNA/GPL complexes hold significant potential for further development. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2011
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