Počet záznamů: 1
Fragmentation of Human Erythrocyte Actin following Exposure to Hypoxia
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SYSNO ASEP 0355472 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Fragmentation of Human Erythrocyte Actin following Exposure to Hypoxia Tvůrce(i) Risso, A. (IT)
Santamaria, B. (CH)
Pistarino, E. (CH)
Cosulich, M. E. (CH)
Pompach, Petr (MBU-M) RID, ORCID
Bezouška, Karel (MBU-M)
Antonutto, G. (IT)Zdroj.dok. Acta Haematologica - ISSN 0001-5792
Roč. 123, č. 1 (2010), s. 6-13Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova beta-Actin ; Erythrocytes ; Hypoxia Vědní obor RIV EE - Mikrobiologie, virologie CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000272340700002 DOI 10.1159/000256661 Anotace In a comparative study on erythrocytes (RBCs) drawn from mountaineers before and after a high-altitude stay, we observed that upon returning to sea level, their RBCs displayed a senescent-like phenotype as indicated by their density and the partial loss of membrane proteins which are shed by ageing RBCs. The aim of this study was to investigate possible changes in the membrane skeleton of these RBCs and to compare them with pathological RBCs. We analysed the proteins of RBC ghosts obtained from our subjects before and after returning to sea level by two-dimensional electrophoresis and mass spectrometry. We observed lower expression and fragmentation of -actin after exposure to hypoxia. This suggested an alteration in membrane skeleton structure, which was confirmed by -actin release in cell lysates during ghost preparation Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2011
Počet záznamů: 1