Počet záznamů: 1
Current Protocols in Chemical Biology
- 1.0353703 - ÚOCHB 2011 RIV US eng M - Část monografie knihy
Macíčková-Cahová, Hana - Vrábel, Milan - Hocek, Michal
Cross-coupling Modification of Nucleoside Triphosphates, PEX, and PCR Construction of Base-Modified DNA.
Current Protocols in Chemical Biology. Vol. 2. New York: Wiley Interscience, 2010, s. 1-14. ISBN 9780470559277
Výzkumný záměr: CEZ:AV0Z40550506
Klíčová slova: nucleoside triphosphates * DNA polymerase * cross-coupling * DNA conjugates
Kód oboru RIV: CC - Organická chemie
A novel, efficient, two-step methodology is presented for construction of base-modified oligonucleotides or DNA involving aqueous cross-coupling reactions of halogenated nucleoside triphosphates (dNTPs) with terminal acetylenes or arylboronic acids, followed by polymerase incorporation of the modified dNTPs either using primer extension (PEX) or polymerase chain reaction (PCR).
Trvalý link: http://hdl.handle.net/11104/0192869
Počet záznamů: 1