Počet záznamů: 1
Conserved mechanism of Wnt signaling function in the specification of vulval precursor fates in C. elegans and C. briggsae
- 1.0352047 - BC 2011 RIV US eng J - Článek v odborném periodiku
Seetharaman, A. - Cumbo, P. - Bojanala, Nagagireesh - Gupta, B. P.
Conserved mechanism of Wnt signaling function in the specification of vulval precursor fates in C. elegans and C. briggsae.
Developmental Biology. Roč. 346, č. 1 (2010), s. 128-139. ISSN 0012-1606. E-ISSN 1095-564X
Výzkumný záměr: CEZ:AV0Z60220518
Klíčová slova: Nematode * C. elegans * C. briggsae * Vulval development * Signal transduction * Wnt signaling
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 4.094, rok: 2010
To understand the function and evolution of Wnt signaling in Caenorhabditis nematodes we focused on C. briggsae, a species that is substantially divergent from C. elegans in terms of the evolutionary time scale yet shares almost identical morphology. We isolated mutants in C briggsae that display multiple pseudo-vulvae resulting from ectopic VPC induction. We cloned one of these loci and found that it encodes an Axin homolog, Cbr-PRY-1. Our genetic studies revealed that Cbr-pry-1 functions upstream of the canonical Wnt pathway components Cbr-bar-1 (beta-catenin) and Cbr-pop-1(tcf/lef) as well as the Hox target Cbr-lin-39 (Dfd/Scr). We further characterized the pry-1 vulval phenotype in C briggsae and C elegans using 8 cell fate markers, cell ablation, and genetic interaction approaches. Our results show that ectopically induced VPCs in pry-1 mutants adopt 2 degrees fates independently of the gonad-derived inductive and LIN-12/Notch-mediated lateral signaling pathways.
Trvalý link: http://hdl.handle.net/11104/0191649
Počet záznamů: 1