Počet záznamů: 1
A microarray for screening the variability of 16S–23S rRNA internal transcribed spacer in Pseudomonas syringae
- 1.0345684 - BC 2011 RIV NL eng J - Článek v odborném periodiku
Lenz, Ondřej - Beran, Pavel - Fousek, Jan - Mráz, Ivan
A microarray for screening the variability of 16S–23S rRNA internal transcribed spacer in Pseudomonas syringae.
Journal of Microbiological Methods. Roč. 82, č. 1 (2010), s. 90-94. ISSN 0167-7012. E-ISSN 1872-8359
Grant CEP: GA ČR GP522/07/P338
Výzkumný záměr: CEZ:AV0Z50510513
Klíčová slova: microarray * ITS1 * mosaicism
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 2.018, rok: 2010
The 16S–23S ribosomal internal transcribed spacer (ITS1) is often used as a subspecies or strain-specific molecular marker for various kinds of bacteria. However, the presence of different copies of ITS1 within a single genome has been reported. Such mosaicism may influence correct typing of many bacteria and therefore knowledge about exact configuration of this region in a particular genome is essential. In order to screen the variability of ITS1 among and within Pseudomonas syringae genomes, an oligonucleotide microarray targeting different configurations of ITS1 was developed. The microarray revealed seven distinct variants in 13 pathovars tested and detected mosaicism within the genomes of P. syringae pv. coronafaciens, pisi, syringae and tabaci. In addition, the findings presented here challenge the using of rRNA analysis for pathovar and strain determination.
Trvalý link: http://hdl.handle.net/11104/0186895
Počet záznamů: 1