Počet záznamů: 1
Identification of molecular targets for selective elimination of TRAIL-resistant leukemia cells. From spots to in vitro assays using TOP15 charts
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SYSNO ASEP 0338760 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Identification of molecular targets for selective elimination of TRAIL-resistant leukemia cells. From spots to in vitro assays using TOP15 charts Tvůrce(i) Petrák, J. (CZ)
Toman, O. (CZ)
Šimonová, T. (CZ)
Halada, Petr (MBU-M) RID, ORCID
Čmejla, R. (CZ)
Klener, P. (CZ)
Živný, J. (CZ)Zdroj.dok. Proteomics. - : Wiley - ISSN 1615-9853
Roč. 9, č. 22 (2009), s. 5006-5015Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Biomedicine ; Drug resistance ; HL-60 Vědní obor RIV CE - Biochemie CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000272391300001 DOI 10.1002/pmic.200900335 Anotace The resistance of malignant cells to chemotherapy calls for the development of novel anti-cancer drugs. TNF-related apoptosis-inducing ligand (TRAIL) is a pro-apoptotic cytokine, which selectively induces apoptosis in malignant cells. We derived two TRAIL-resistant HL-60 subclones, HL-60/P1 and HL-60/P2, from a TRAIL-sensitive HL-60 acute promyelocytic leukemia cell line. To identify therapeutically exploitable weaknesses of the TRAIL-resistant leukemia cells that could be used as molecular targets for their elimination, we performed proteomic (2-DE) analysis and compared both TRAIL-resistant subclones with the original TRAIL-sensitive HL-60 cells. We identified over 40 differentially expressed proteins. To significantly narrow the lists of candidate proteins, we excluded proteins that are known to be often differentially expressed, regardless of experiment type and tissue (the so-called TOP15 proteins) Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2010
Počet záznamů: 1