Počet záznamů: 1
Outcome of Treatment of Human HeLa Cervical Cancer Cells With Roscovitine Strongly Depends on the Dosage and Cell Cycle Status Prior to the Treatment
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SYSNO ASEP 0337483 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Outcome of Treatment of Human HeLa Cervical Cancer Cells With Roscovitine Strongly Depends on the Dosage and Cell Cycle Status Prior to the Treatment Tvůrce(i) Wesierska-Gadek, J. (AT)
Borza, A. (AT)
Walzi, E. (AT)
Kryštof, Vladimír (UEB-Q) RID, ORCID
Maurer, M. (AT)
Komina, O. (AT)
Wandl, S. (AT)Zdroj.dok. Journal of Cellular Biochemistry. - : Wiley - ISSN 0730-2312
Roč. 106, č. 5 (2009), s. 937-955Poč.str. 19 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova APOPTOSIS ; CELL CYCLE ARREST ; CYCLIN-DEPENDENT KINASES Vědní obor RIV ED - Fyziologie CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000264664800022 DOI 10.1002/jcb.22074 Anotace Exposure of asynchronously growing human HeLa cervical carcinoma cells to roscovitine (ROSC), a selective cyclin-dependent kinases (CDKs) inhibitor, arrests their progression at the transition between G(2)/M and/or induces apoptosis. The outcome depends on the ROSC concentration. At higher close ROSC represses HPV-encoded E7 oncoprotein and initiates caspase-dependent apoptosis. Inhibition of the site-specific phosphorylation of survivin and Bad, occurring at high-dose ROSC treatment, precedes the onset of apoptosis and seems to be a prerequisite for cell death. Considering the fact that in HeLa cells the G(1)/S restriction checkpoint is abolished by E7, we addressed the question whether the inhibition of CDKs by pharmacological inhibitors in synchronized cells would be able to block the cell-cycle in G, phase. For this purpose, we attempted to synchronize cells by serum withdrawal or by blocking of the mitotic apparatus using nocodazole. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2010
Počet záznamů: 1