Počet záznamů: 1
Head and neck squamous cancer stromal fibroblasts produce growth factors influencing phenotype of normal human keratinocytes
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SYSNO ASEP 0333778 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Head and neck squamous cancer stromal fibroblasts produce growth factors influencing phenotype of normal human keratinocytes Tvůrce(i) Strnad, Hynek (UMG-J) RID
Lacina, L. (CZ)
Kolář, Michal (UMG-J) RID, ORCID
Čada, Z. (CZ)
Vlček, Čestmír (UMG-J) RID
Dvořánková, B. (CZ)
Betka, J. (CZ)
Plzák, J. (CZ)
Chovanec, M. (CZ)
Šáchová, Jana (UMG-J)
Valach, J. (CZ)
Urbanová, Markéta (UMG-J)
Smetana, K. Jr. (CZ)Celkový počet autorů 13 Zdroj.dok. Histochemistry and Cell Biology. - : Springer - ISSN 0948-6143
Roč. 133, č. 2 (2010), s. 201-211Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Cancer microenvironment ; Epithelial–mesenchymal interaction ; Cancer-associated fibroblasts Vědní obor RIV EB - Genetika a molekulární biologie CEP 2B06106 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000273745800006 DOI 10.1007/s00418-009-0661-6 Anotace Epithelial–mesenchymal interaction between stromal fibroblasts and cancer cells influences the functional properties of tumor epithelium, including the tumor progression and spread. We compared fibroblasts prepared from stroma of squamous cell carcinoma and normal dermal fibroblasts concerning their biological activity toward normal keratinocytes assessed by immunocytochemistry and profiling of gene activation for growth factors/cytokines by microarray chip technology. IGF-2 and BMP-4 were determined as candidate factors responsible for tumor-associated fibroblast activity that influences normal epithelia. This effect was confirmed by addition of recombinant IGF-2 and BMP4, respectively, to the culture medium. This hypothesis was also verified by inhibition experiments where blocking antibodies were employed in the medium conditioned by cancer-associated fibroblast. Presence of these growth factors was also detected in tumor samples. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2010
Počet záznamů: 1