0312545 - MBÚ 2009 RIV US eng J - Článek v odborném periodiku
Szamecz, Bela - Rutkai, Edit - Cuchalová, Lucie - Munzarová, Vanda - Herrmannová, Anna - Nielsen, K. H. - Burela, L. - Hinnebusch, A. G. - Valášek, Leoš
eIF3a cooperates with sequences 5´of uORF1 to promote resumption of scanning by post-termination ribosomes for reinitiation on GCN4 mRNA.
[eIF3 hraje kritickou úlohu v procesu reiniciace, který řídí expresi kvasinkového genu GCN4, prostřednictvým interakce s 5’ oblastí uORF1 nacházející se v netranslatované oblasti GCN4.]
Genes and Development. Roč. 22, - (2008), s. 2414-2425. ISSN 0890-9369. E-ISSN 1549-5477
Grant ostatní: GB(GB) The Wellcome Trusts Grant 076456/Z/05/Z; US(US) NIH Research Grant R01 TW007271
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: translation initiation * reinitiation * eif3
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 13.623, rok: 2008 ; AIS: 9.105, rok: 2008
DOI: https://doi.org/10.1101/gad.480508

Yeast initiation factor eIF3 (eukaryotic initiation factor 3) has been implicated in multiple steps of translation initiation. Previously, we showed that the N-terminal domain (NTD) of eIF3a interacts with the small ribosomal protein RPS0A located near the mRNA exit channel, where eIF3 is proposed to reside. Here, we demonstrate that a partial deletion of the RPS0A-binding domain of eIF3a impairs translation initiation and reduces binding of eIF3 and associated eIFs to native preinitiation complexes in vivo. Strikingly, it also severely blocks the induction of GCN4 translation that occurs via reinitiation. Detailed examination unveiled a novel reinitiation defect resulting from an inability of 40S ribosomes to resume scanning after terminating at the first upstream ORF (uORF1). Genetic analysis reveals a functional interaction between the eIF3a-NTD and sequences 5_ of uORF1 that is critically required to enhance reinitiation

Práce pojednává o studiu iniciace translace a specifické úloze translačního iniciačního faktoru eIF3
Trvalý link: http://hdl.handle.net/11104/0163583