Počet záznamů: 1
Impact of Two-step In Vitro Culture Systems on Developmental Potency of Oocytes
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SYSNO ASEP 0213221 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Impact of Two-step In Vitro Culture Systems on Developmental Potency of Oocytes Tvůrce(i) Motlík, Jan (UZFG-Y) RID, ORCID
Pavlok, Antonín (UZFG-Y) RID
Lapathitis, Georgios (UZFG-Y)
Kubelka, Michal (UZFG-Y) RID, ORCIDZdroj.dok. Reproduction in Domestical Animals. - : Wiley - ISSN 0936-6768
Roč. 35, - (2000), s. 267-271Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Vědní obor RIV EB - Genetika a molekulární biologie CEP GV524/96/K162 GA ČR - Grantová agentura ČR KSK2038602 GA AV ČR - Akademie věd Anotace Proper conditions for the successful induction of a functional two step in vitro culture system for agricultural or clinical applications are hereby described in form of a two-step protocol. The first inhibitory culture of oocytes in media supplemented with specific cdk inhibitors (roscovitine, BL I) appears essential to promote appropriate oocyte-cumulus cell interactions and ensure production of ova competent to undergo normal embryogenesis. This inhibitory period should last for several days, depending on the origin of the follicles, and the degree of accumulation and storage of mRNAs and proteins essential for the acquisition of developmental competence in the oocytes. The full reversibility of the inhibitory step secures that during the second maturation step, oocytes reach metaphase IT with correctly arranged microtubuli in the meiotic spindle and well-expanded cumuli. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2001
Počet záznamů: 1