Počet záznamů: 1
Polyclonal Antibodies to a Recombinant Coat Protein of Potato Virus A
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SYSNO ASEP 0172249 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Polyclonal Antibodies to a Recombinant Coat Protein of Potato Virus A Tvůrce(i) Čeřovská, Noemi (UEB-Q) RID, ORCID
Moravec, Tomáš (UEB-Q) RID, ORCID
Velemínský, Jiří (UEB-Q)Zdroj.dok. Acta virologica - ISSN 0001-723X
Roč. 46, - (2002), s. 147-151Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova Potato virus A ; recombinant coat protein ; Escherichia coli Vědní obor RIV EB - Genetika a molekulární biologie CEP GA310/00/0381 GA ČR - Grantová agentura ČR CEZ AV0Z5038910 - UEB-Q Anotace Specific mouse antibodies against a recombinant coat protein (CP) of Potato virus A (PVA) were produced. The PVA CP gene was cloned in an expression vector. After expression in Escherichia coli the presence of the expressed CP was proved by Western blot analysis using polyclonal and monoclonal antibodies (MAbs). The expressed CP was purified by centrifugation in CsCl density gradient or on a sucrose cushion. The production of virus-like particles (VLPs) was proved by electron microscopy. The purified CP was used for preparation of a mouse antiserum which had a liter of 1:1024 in ELIS A and reacted specifically in Western blot analysis and indirect plate-trapped antigen ELISA (PTA-ELISA). Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2003
Počet záznamů: 1