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Dynamics of gel isoelectric focusing with ampholytic dyes monitored by camera in real-time
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SYSNO ASEP 0162475 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Dynamics of gel isoelectric focusing with ampholytic dyes monitored by camera in real-time Tvůrce(i) Šťastná, Miroslava (UIACH-O) RID, ORCID
Šlais, Karel (UIACH-O) RID, ORCIDZdroj.dok. Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1008, č. 2 (2003), s. 193-203Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Dynamics ; gel isoelectric focusing ; pI markers Vědní obor RIV CB - Analytická chemie, separace CEP IBS4031201 GA AV ČR - Akademie věd GA203/02/1447 GA ČR - Grantová agentura ČR CEZ AV0Z4031919 - UIACH-O Anotace The dynamics of gel isoelectric focusing were studied by using amphoteric low-molecular-mass colored substances (isoelectric point markers).The polyacrylamide gel in slab format was in direct contact with the electrodes. In addition to isoelectric focusing with pH gradient composed of synthetic carrier ampholytes, pH gradients created by simple buffers were applied. The progress of the elerctrofocusing process was monitored by CCD camera and video recording. The gradient profile and dynamics were approximated from the positions of isoelectric point markers. The obtained animated records enabled the observation of the entire real focusing run within fractions of a minute, which is useful both for the understanding and optimization of the focusing. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2004
Počet záznamů: 1