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Dynamic modification of proteins for fluorometric detection in CZE
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SYSNO ASEP 0162299 Druh ASEP C - Konferenční příspěvek (mezinárodní konf.) Zařazení RIV D - Článek ve sborníku Název Dynamic modification of proteins for fluorometric detection in CZE Tvůrce(i) Horká, Marie (UIACH-O) RID, ORCID
Šlais, Karel (UIACH-O) RID, ORCIDZdroj.dok. 2nd International Symposium Separations in the BioSciences. SBS 2001. - Praha, 2001 - ISBN 80-7080-437-8 Rozsah stran s. 91 Poč.str. 1 s. Akce International Symposium Separations in the BioSciences /2./ Datum konání 17.09.2001-20.09.2001 Místo konání Praha Země CZ - Česká republika Typ akce EUR Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova capillary zone electrophoresis ; fluorometric detection ; dynamic modification Vědní obor RIV CB - Analytická chemie, separace CEP IAA4031901 GA AV ČR - Akademie věd CEZ AV0Z4031919 - UIACH-O Anotace The separation techniques employing fluorescence detection are sensitive and selective so they have been often applied for the trace analysis of biological samples. The commonly used derivatization of proteins can lower the detection limits; however, they can change the acido-basic properties and mobilities when compared to the native species. Recently, we have used the colored tenside as a buffer additive for the photometric detection of proteins in the UV region. The selectivity, efficiency and resolution of CZE separation using this dye were found to be similar to the CZE with SDS as the additive. In this study, the ampiphilic fluorescent compound is suggested as a buffer additive in CZE for dynamic modification of the sample of several proteins. Using the deuterium lamp for the excitation in the UV region for the on column fluorometric detection, the amol minimum detectable amounts of the proteins sampled on the CZE capillary were achieved. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2002
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