Počet záznamů: 1
Rapid capillary coating by epoxy-poly-(dimethylacrylamide): Performance in capillary zone electrophoresis of protein and polystyrene carboxylate
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SYSNO ASEP 0162266 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Rapid capillary coating by epoxy-poly-(dimethylacrylamide): Performance in capillary zone electrophoresis of protein and polystyrene carboxylate Tvůrce(i) Chiari, M. (IT)
Cretich, M. (IT)
Šťastná, Miroslava (UIACH-O) RID, ORCID
Radko, S. P. (US)
Chrambach, A. (US)Zdroj.dok. Electrophoresis. - : Wiley - ISSN 0173-0835
Roč. 22, č. 4 (2001), s. 656-659Poč.str. 4 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova capillary coating ; capillary zone electrophoresis ; proteins Vědní obor RIV CB - Analytická chemie, separace CEZ AV0Z4031919 - UIACH-O Anotace A fast and simple method for the internal coating of capillaries in capillary zone electrophoresis (CZE) is that with epoxy-poly(dimethylacrylamide) (EPDMA), Duration of coating by that method is 30 min, compared with that of 24 h when using uncross-linked polyacrylamide (PA) under otherwise identical conditions. Under the conditions used for the CZE of proteins (pH 9.0, 2% polyethylene glycol), the capillary coating with EPDMA is stable for at least 50 consecutive runs as judged by the constancy of low electroosmotic flow, equalling the stability of coating achieved by PA. Protein mobilities and protein peak asymmetry (suggestive of reversible interaction with the capillary wall) are also found to be the same in EPDMA and PA coated capillaries. Differences between EPDMA and PA coating also exist. The former is unstable upon lowering the ionic strength of the buffer to 0.003, upon the addition of sodium dodecyl sulfate (SDS) to the buffer and in application to the hydrophobic analyte, polystyrene carboxylate. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2002
Počet záznamů: 1