Počet záznamů: 1  

Dual effects of muscarinic M2 receptors on the synthesis of cyclic AMP in CHO cells: background and model

  1. 1.
    SYSNO ASEP0142134
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JOstatní články
    NázevDual effects of muscarinic M2 receptors on the synthesis of cyclic AMP in CHO cells: background and model
    Tvůrce(i) Tuček, Stanislav (FGU-C)
    Michal, Pavel (FGU-C) RID
    Vlachová, Viktorie (FGU-C) RID, ORCID, SAI
    Zdroj.dok.Life Sciences. - : Elsevier - ISSN 0024-3205
    Roč. 68, 22-23 (2001), s. 2501-2510
    Poč.str.10 s.
    Jazyk dok.eng - angličtina
    Země vyd.GB - Velká Británie
    Klíč. slovamuscarinic receptors ; G proteins ; adenylyl cyclase
    Vědní obor RIVED - Fyziologie
    CEPIAA7011910 GA AV ČR - Akademie věd
    GA309/99/0214 GA ČR - Grantová agentura ČR
    CEZAV0Z5011922 - FGU-C
    AnotaceIt has been observed in several laboratories that muscarinic agonists have dual effects on the synthesis of cyclic AMP in cell lines expressing muscarinic M2 or M4 receptors, producing strong inhibition at low agonist concentrations and lesser inhibition or stimulation at high agonist concentrations. Data obtained on CHO cells (known to express adenylyl cyclases VI and VII) are best interpreted on the assumption that the upward phase of the concentration-response curves reflects simultaneous inhibition of adenylyl cyclase VI via the Gi proteins, with which the M2 and M4 receptors communicate with high affinity, and stimulation of adenylyl cyclases VI and VII via the Gs proteins, with which the M2 and M4 receptors communicate with low affinity. A simplified model is described which permits one to predict how the shapes of the concentration-response curves will be affected by changes in the concentration of receptors, the affinities of activated receptors for Gi or Gs proteins, and other parameters.
    PracovištěFyziologický ústav
    KontaktLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Rok sběru2002

Počet záznamů: 1  

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