Genetic polymorphism within the Leishmania donovani complex: correlation with geographic origin
1.
SYSNO ASEP
0104486
Druh ASEP
J - Článek v odborném periodiku
Zařazení RIV
J - Článek v odborném periodiku
Poddruh J
Ostatní články
Název
Genetic polymorphism within the Leishmania donovani complex: correlation with geographic origin
Překlad názvu
Genetický polymorfismus uvnitř Leishmania donovani komplexu koreluje s jejich geografickým původem
Tvůrce(i)
Zemanová, Eva (PAU-O) Jirků, Milan (PAU-O) Mauricio, I. L. (GB) Miles, M. A. (GB) Lukeš, Julius (PAU-O)
Zdroj.dok.
American Journal of Tropical Medicine and Hygiene
- ISSN 0002-9637
Roč. 70, č. 6 (2004), s. 613-617
Poč.str.
5 s.
Jazyk dok.
eng - angličtina
Země vyd.
US - Spojené státy americké
Klíč. slova
genetic polymorphism ; Leishmania donovani ; RAPD
Vědní obor RIV
EB - Genetika a molekulární biologie
CEZ
AV0Z6022909 - PAU-O, BC-A
Anotace
Random amplified polymorphic DNA (RAPD) was used to detect intraspecific diversity for the Leishmania donovani complex. Fifty-two decameric to 21-meric primers of arbitrary sequence were applied to 15 strains that belong to nine zymodemes. Strains belonging to the species L. major and L. tropica were used as outgroups. A total of 902 amplicons generated by RAPD were scored. Most primers produced species-specific profiles, only 0.6% amplicons were shared by all species, while 4.3% amplicons were common for all 15 strains of the L. donovani complex. Well-supported trees have been constructed, which show a rather strong correlation between the genetic polymorphism of studied strains and their geographic origin. In all obtained trees, L. infantum was paraphyletic. The RAPD profiles suggest that MON-30 belongs to L. donovani. Moreover, the genetic distance between the L. archibaldi strain and other leishmanias does not warrant existence of a separate species
