Random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphisms (AFLP) were used to survey genetic variability among 140 isolates of Claviceps africana collected from Southern Africa, India, Thailand, Australia, and the Americas in 1992-2002. In the Americas, a single RAPD haplotype was found throughout the various countries. The Eastern lineage consisted of two close haplotypes (one from India, the other from Thailand and Australia)