Phylogenetic analysis of freshwater fish trypanosomes from Europe using ssu rRNA gene sequences and random amplification of polymorphic DNA
1.
SYSNO ASEP
0025690
Druh ASEP
J - Článek v odborném periodiku
Zařazení RIV
J - Článek v odborném periodiku
Poddruh J
Ostatní články
Název
Phylogenetic analysis of freshwater fish trypanosomes from Europe using ssu rRNA gene sequences and random amplification of polymorphic DNA
Překlad názvu
Fylogenetická analýza trypanosom sladkovodních ryb Evropy využívající sekvence ssu rRNA genu a polymorfismus náhodně amplifikované DNA
Tvůrce(i)
Gibson, W. C. (GB) Lom, Jiří (PAU-O) Pecková, Hana (PAU-O) Ferris, V. R. (GB) Hamilton, P. B. (GB)
Zdroj.dok.
Parasitology. - : Cambridge University Press
- ISSN 0031-1820
Roč. 130, č. 4 (2005), s. 405-412
Poč.str.
8 s.
Jazyk dok.
eng - angličtina
Země vyd.
GB - Velká Británie
Klíč. slova
trypanosomes ; freshwater fish ; phylogeny
Vědní obor RIV
EA - Morfologické obory a cytologie
CEZ
AV0Z60220518 - PAU-O, BC-A (2005-2011)
Anotace
A collection of 22 cloned trypanosome isolates from 14 species of European freshwater fish and 1 species of African freshwater fish were examined by phylogenetic analysis. SSU rRNA genes of 8 isolates were sequenced and compared with sequences from a wide selection of vertebrate trypanosome isolates. All freshwater isolates fell in a single clade, subdivided into 3 groups. This clade sits within a robust clade of trypanosomes from marine fish, amphibia, reptiles and the duck-billed platypus. All 22 trypanosome clones were analysed by RAPD. The resulting tree shows 3 groups congruent with the groups identified in the ssu rRNA gene phylogeny. Two of these groups contain the majority of isolates. These groups do not separate trypanosome species distinguished by morphology or host origin, and thus these criteria do not appear to be reliable guides to genetic relationships among fish trypanosomes. The two groups themselves may represent different species of fish trypanosomes.
