| SYSNO ASEP | 0025664
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| Druh ASEP | J - Článek v odborném periodiku
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| Zařazení RIV | J - Článek v odborném periodiku
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| Poddruh J | Ostatní články
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| Název | RNA interference analyses suggest a transcript-specific regulatory role for mitochondrial RNA-binding proteins MRP1 and MRP2 in RNA editing and other RNA processing in Trypanosoma brucei
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| Překlad názvu | Analýza prostřednictvím interference RNA ukazuje na transkripčně specifickou úlohu mitochondriálních RNA-vážících proteinů MRP1 a MRP2 při editování RNA a dalších úpravách RNA u Trypanosoma brucei
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| Tvůrce(i) | Horáková, Eva (PAU-O)
Van Den Burg, J. (NL)
Zíková, Alena (PAU-O)
Ernst, N. L. (US)
Stuart, K. (US)
Benne, R. (NL)
Lukeš, Julius (PAU-O)
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| Zdroj.dok. | Journal of Biological Chemistry. - : Elsevier
- ISSN 0021-9258
Roč. 280, č. 4 (2005), s. 2429-2438
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| Poč.str. | 10 s.
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| Jazyk dok. | eng - angličtina
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| Země vyd. | US - Spojené státy americké
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| Klíč. slova | Trypanosoma brucei ; RNA editing ; interference RNA
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| Vědní obor RIV | EB - Genetika a molekulární biologie
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| CEP | IAA6022903 GA AV ČR - Akademie věd
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| CEZ | AV0Z60220518 - PAU-O, BC-A (2005-2011)
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| Anotace | Mitochondrial RNA binding proteins MRP1 and MRP2 occur in a heteromeric complex that appears to play a role in U-insertion/deletion editing in trypanosomes. Reduction in the levels of MRP 1 (gBP21) and/or MRP2 (gBP25) mRNA by RNA interference (RNAi) in procyclic Trypanosoma brucei resulted in severe growth inhibition. It also resulted in the loss of both proteins, even when only one of the MRPs mRNAs was reduced, indicating a mutual dependence for stability. Elimination of the MRPs gave rise to substantially reduced levels of edited cyB and RPS12 mRNAs, but little or no reduction of the level of edited cox2, cox3 and A6 mRNAs, as measured by poisoned primer extension analyses. In contrast, edited ND7 mRNA was increased five-fold in MRP1+2 double knockdown cells. These data indicate that MRP1 and MRP2 are not essential for RNA editing per se, but rather play a regulatory role in the editing of specific transcripts and other RNA processing activities.
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| Pracoviště | Parazitologický ústav (do r. 2005)
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| Kontakt | Kateřina Homrová,katerina.homrova@paru.cas.cz , Tel.: 387 775 406
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| Rok sběru | 2006
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