Počet záznamů: 1
Expression and thermotolerance of calreticulin during pollen development in tobacco
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SYSNO ASEP 0024804 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Expression and thermotolerance of calreticulin during pollen development in tobacco Překlad názvu Exprese a tepelná odolnost calreticulinu během vývoje pylu tabáku Tvůrce(i) Hrubá, Petra (UEB-Q)
Honys, David (UEB-Q) RID, ORCID
Tupý, Jaroslav (UEB-Q) RIDZdroj.dok. Sexual Plant Reproduction - ISSN 0934-0882
Roč. 18, č. 3 (2005), s. 143-148Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova tobacco ; pollen development ; thermotolerance Vědní obor RIV EB - Genetika a molekulární biologie CEP GP522/02/D075 GA ČR - Grantová agentura ČR LZ1K03018 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy KJB6038409 GA AV ČR - Akademie věd CEZ AV0Z50380511 - UEB-Q (2005-2011) Anotace Glycoproteins 50, 55, 59 and 64 kDa with affinity to the lectin ConA occurring abundantly in mature tobacco pollen were shown to exhibit high tolerance against heating at 90 °C for 30 min. The 59 kDa glycoprotein (GP59) was isolated by affinity chromatography on ConA-agarose followed by 2D-electrophoresis and identified by MS analysis as tobacco calreticulin with approximate pI 4.2. Identification of the protein was confirmed by immunoblotting with human anti-calreticulin and by labeling with a specific dye for Ca2+ binding proteins (Stains All). Two acidic isoforms of 50 kDa glycoprotein, in addition to GP59, displayed homology to calreticulin. RT-PCR revealed presence of transcripts for calreticulin 59 kDa at all stages of pollen development from microspore mitosis throughout the first 24 h after in vitro pollen germination. Immunodetection with anti-calreticulin and affinity to ConA on Western blots of total soluble proteins separated by 1D-SDS-PAGE, the protein first occurred at the mid-bicellular pollen stage, accumulated during pollen maturation and disappeared during the first 24 h after pollen germination. The thermotolerant protein GP59 was detected only in the terminal phase of pollen maturation and during the first 8 h of pollen germination. Results indicated that calreticulin 59 kDa is transcribed, translated and undergoes posttranslational modification at a number of different stages of pollen development and that thermotolerance of the protein in mature pollen may be associated with high glycosylation. Thermotolerance of these glycoproteins could play a role in pollen protection against stress factors during dehydration and dispersal. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2006
Počet záznamů: 1