0588254 - MBÚ 2025 RIV GB eng J - Článek v odborném periodiku
Kalkusová, K. - Táborská, P. - Stakheev, D. L. - Rataj, J. - Smite, S. - Darras, E. - Albo, J. - Bartůňková, J. - Vannucci, Luca - Smrž, Daniel
Impaired Proliferation of CD8+T Cells Stimulated with Monocyte-Derived Dendritic Cells Previously Matured with Thapsigargin-Stimulated LAD2 Human Mast Cells.
Journal of Immunology Research. Roč. 2024, July 18 (2024), č. článku 5537948. ISSN 2314-8861. E-ISSN 2314-7156
Grant CEP: GA MZd(CZ) NU23-08-00071
Institucionální podpora: RVO:61388971
Klíčová slova: fc-epsilon-ri * tnf-alpha * lung-cancer * activation * maturation * il-12 * immature * immunity * naive * mechanism
Obor OECD: Immunology
Impakt faktor: 3.5, rok: 2023 ; AIS: 0.884, rok: 2023
Způsob publikování: Open access
Web výsledku:
https://onlinelibrary.wiley.com/doi/10.1155/2024/5537948DOI: https://doi.org/10.1155/2024/5537948

CD8(+) T cells are essential for adaptive immunity against infection and tumors. Their ability to proliferate after stimulation is crucial to their functionality. Dendritic cells (DCs) are professional antigen-presenting cells that induce their proliferation. Here, we show that thapsigargin-induced LAD2 mast cell (MC) line-released products can impair the ability of monocyte-derived DCs to induce CD8(+) T-cell proliferation and the generation of Th1 cytokine-producing T cells. We found that culture medium conditioned with LAD2 MCs previously stimulated with thapsigargin (thapsLAD2) induces maturation of DCs as determined by the maturation markers CD80, CD83, CD86, and HLA-DR. However, thapsLAD2-matured DCs produced no detectable TNF alpha or IL-12 during the maturation. In addition, although their surface expression of PD-L1 was comparable with the immature or TLR7/8-agonist (R848)-matured DCs, their TIM-3 expression was significantly higher than in immature DCs and even much higher than in R848-matured DCs. In addition, contrary to R848-matured DCs, the thapsLAD2-matured DCs only tended to induce enhanced proliferation of CD4+ T cells than immature DCs. For CD8(+) T cells, this tendency was not even detected because thapsLAD2-matured and immature DCs comparably induced their proliferation, which contrasted with the significantly enhanced proliferation induced by R848-matured DCs. Furthermore, these differences were comparably recapitulated in the ability of the tested DCs to induce IFN gamma- and IFN gamma/TNF alpha-producing T cells. These findings show a novel mechanism of MC-mediated regulation of adaptive immune responses.
Trvalý link: https://hdl.handle.net/11104/0355298