Počet záznamů: 1
Adipose-Derived Stem Cells in Reinforced Collagen Gel: A Comparison between Two Approaches to Differentiation towards Smooth Muscle Cells
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SYSNO ASEP 0570713 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Adipose-Derived Stem Cells in Reinforced Collagen Gel: A Comparison between Two Approaches to Differentiation towards Smooth Muscle Cells Tvůrce(i) Filová, Elena (FGU-C) RID, ORCID
Šupová, Monika (USMH-B) RID, ORCID, SAI
Eckhardt, Adam (FGU-C) RID, ORCID
Vrbacký, Marek (FGU-C) RID, ORCID
Blanquer, Andreu (FGU-C) RID, ORCID
Trávníčková, Martina (FGU-C) RID, ORCID, SAI
Knitlová, Jarmila (FGU-C) RID, ORCID
Suchý, Tomáš (USMH-B) RID, ORCID, SAI
Rýglová, Šárka (USMH-B) RID, ORCID, SAI
Braun, Martin (USMH-B) ORCID, SAI
Burdíková, Z. (CZ)
Schätz, M. (CZ)
Jenčová, V. (CZ)
Lisnenko, M. (CZ)
Běhálek, L. (CZ)
Procházková, R. (CZ)
Sedláček, R. (CZ)
Kubášová, K. (CZ)
Bačáková, Lucie (FGU-C) RID, ORCIDČíslo článku 5692 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 24, č. 6 (2023)Poč.str. 29 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova collagen particles ; stem cells differentiation ; vascular patches ; adipose tissue-derived stem cells ; gel reinforcement ; endothelial cells ; extracellular matrix ; remodelling ; tissue engineering Obor OECD Endocrinology and metabolism (including diabetes, hormones) CEP NV19-02-00068 GA MZd - Ministerstvo zdravotnictví NV18-01-00332 GA MZd - Ministerstvo zdravotnictví LX22NPO5104 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF18_046/0016045 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura e-INFRA CZ - 90140 - CESNET, zájmové sdružení právnických osob
Czech-BioImaging II - 90129 - Ústav molekulární genetiky AV ČR, v. v. i.Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 ; USMH-B - RVO:67985891 UT WOS 000958331700001 EID SCOPUS 85151107188 DOI 10.3390/ijms24065692 Anotace Scaffolds made of degradable polymers, such as collagen, polyesters or polysaccharides, are promising matrices for fabrication of bioartificial vascular grafts or patches. In this study, collagen isolated from porcine skin was processed into a gel, reinforced with collagen particles and with incorporated adipose tissue-derived stem cells (ASCs). The cell-material constructs were then incubated in a DMEM medium with 2% of FS (DMEM_part), with added polyvinylalcohol nanofibers (PVA_part sample), and for ASCs differentiation towards smooth muscle cells (SMCs), the medium was supplemented either with human platelet lysate released from PVA nanofibers (PVA_PL_part) or with TGF-beta 1 + BMP-4 (TGF + BMP_part). The constructs were further endothelialised with human umbilical vein endothelial cells (ECs). The immunofluorescence staining of alpha-actin and calponin, and von Willebrand factor, was performed. The proteins involved in cell differentiation, the extracellular matrix (ECM) proteins, and ECM remodelling proteins were evaluated by mass spectrometry on day 12 of culture. Mechanical properties of the gels with ASCs were measured via an unconfined compression test on day 5. Gels evinced limited planar shrinkage, but it was higher in endothelialised TGF + BMP_part gel. Both PVA_PL_part samples and TGF + BMP_part samples supported ASC growth and differentiation towards SMCs, but only PVA_PL_part supported homogeneous endothelialisation. Young modulus of elasticity increased in all samples compared to day 0, and PVA_PL_part gel evinced a slightly higher ratio of elastic energy. The results suggest that PVA_PL_part collagen construct has the highest potential to remodel into a functional vascular wall. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2024 Elektronická adresa https://www.mdpi.com/1422-0067/24/6/5692
Počet záznamů: 1