Počet záznamů: 1
Sex chromosome differentiation via changes in the Y chromosome repeat landscape in African annual killifishes Nothobranchius furzeri and N. kadleci
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SYSNO ASEP 0564193 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Sex chromosome differentiation via changes in the Y chromosome repeat landscape in African annual killifishes Nothobranchius furzeri and N. kadleci Tvůrce(i) Štundlová, Jana (UZFG-Y)
Hospodářská, M. (CZ)
Lukšíková, Karolína (UZFG-Y)
Voleníková, Anna (UZFG-Y)
Pavlica, Tomáš (UZFG-Y)
Altmanová, Marie (UZFG-Y) ORCID
Richter, A. (DE)
Reichard, Martin (UBO-W) RID, ORCID, SAI, SAI
Dalíková, Martina (BC-A) RID, ORCID
Pelikánová, Šárka (UZFG-Y)
Marta, Anatolie (UZFG-Y) ORCID
Simanovsky, S. A. (RU)
Hiřman, Matyáš (UZFG-Y)
Jankásek, Marek (UZFG-Y)
Dvořák, Tomáš (UZFG-Y) ORCID
Bohlen, Jörg (UZFG-Y) ORCID
Ráb, Petr (UZFG-Y) RID, ORCID
Englert, C. (DE)
Nguyen, Petr (UZFG-Y)
Sember, Alexandr (UZFG-Y) RID, ORCIDZdroj.dok. Chromosome Research. - : Springer - ISSN 0967-3849
Roč. 30, č. 4 (2022), s. 309-333Poč.str. 25 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova inversion ; recombination suppression ; sex chromosome degeneration Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Genetics and heredity (medical genetics to be 3) CEP GJ19-22346Y GA ČR - Grantová agentura ČR EF15_003/0000460 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2018131 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora UZFG-Y - RVO:67985904 ; BC-A - RVO:60077344 ; UBO-W - RVO:68081766 UT WOS 000865146200001 EID SCOPUS 85139649638 DOI 10.1007/s10577-022-09707-3 Anotace Homomorphic sex chromosomes and their turnover are common in teleosts. We investigated the evolution of nascent sex chromosomes in several populations of two sister species of African annual killifishes, Nothobranchius furzeri and N. kadleci, focusing on their under-studied repetitive landscape. We combined bioinformatic analyses of the repeatome with molecular cytogenetic techniques, including comparative genomic hybridization, fluorescence in situ hybridization with satellite sequences, ribosomal RNA genes (rDNA) and bacterial artificial chromosomes (BACs), and immunostaining of SYCP3 and MLH1 proteins to mark lateral elements of synaptonemal complexes and recombination sites, respectively. Both species share the same heteromorphic XY sex chromosome system, which thus evolved prior to their divergence. This was corroborated by sequence analysis of a putative master sex determining (MSD) gene gdf6Y in both species. Based on their divergence, differentiation of the XY sex chromosome pair started approximately 2 million years ago. In all populations, the gdf6Y gene mapped within a region rich in satellite DNA on the Y chromosome long arms. Despite their heteromorphism, X and Y chromosomes mostly pair regularly in meiosis, implying synaptic adjustment. In N. kadleci, Y-linked paracentric inversions like those previously reported in N. furzeri were detected. An inversion involving the MSD gene may suppress occasional recombination in the region, which we otherwise evidenced in the N. furzeri population MZCS-121 of the Limpopo clade lacking this inversion. Y chromosome centromeric repeats were reduced compared with the X chromosome and autosomes, which points to a role of relaxed meiotic drive in shaping the Y chromosome repeat landscape. We speculate that the recombination rate between sex chromosomes was reduced due to heterochiasmy. The observed differences between the repeat accumulations on the X and Y chromosomes probably result from high repeat turnover and may not relate closely to the divergence inferred from earlier SNP analyses. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2023 Elektronická adresa https://link.springer.com/article/10.1007/s10577-022-09707-3
Počet záznamů: 1